Other

Part:BBa_K2983011:Design

Designed by: Tom Zaplana   Group: iGEM19_Evry_Paris-Saclay   (2019-10-11)


Loop F-Beta


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 9
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal SapI.rc site found at 17


Design Notes

BsaI with CGCT overhang

SapI with GCA overhang


Source

DNA synthesis

References